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Contents

9

Biodegradation of Plastics by Microorganisms

123

Md. Anisur R. Mazumder, Md. Fahad Jubayer, and Thottiam V. Ranganathan

9.1

Introduction

123

9.2

Definition and Classification of Plastics

124

9.2.1

Definition of Plastic

124

9.2.2

Classification

125

9.2.2.1

Based on Biodegradability

125

9.2.2.2

Based on Structure and Thermal Properties

126

9.2.2.3

Characteristics of Different Biodegradable Plastics

126

9.3

Biodegradation of Plastics

128

9.3.1

General Outline

128

9.3.2

Biodegradation Phases and End Products

129

9.3.2.1

Aerobic Biodegradation

129

9.3.2.2

Anaerobic Biodegradation

130

9.3.3

Mechanism of Microbial Degradation of Plastic

130

9.3.4

Factors Affecting Biodegradation of Plastics

131

9.3.5

Microorganisms Involved in the Biodegradation Process

132

9.3.6

Enzymes Involved in the Plastic Biodegradation

133

9.3.6.1

Cutinases (EC 3.1.1.74)

135

9.3.6.2

Lipases (EC 3.1.1.3)

135

9.3.6.3

Carboxylesterases (EC 3.1.1.1)

135

9.3.6.4

Proteases

135

9.3.6.5

Lignin Modifying Enzymes

136

9.4

Current Trends and Future Prospects

136

List of Abbreviations

137

References

138

10

Enzyme Technology for the Degradation of Lignocellulosic

Waste

143

Swarrna Haldar and Soumitra Banerjee

10.1

Introduction

143

10.2

Enzymes Required for the Degradation of Lignocellulosic Waste

144

10.2.1

Degradation of Cellulose

144

10.2.1.1

Microbial Production of Cellulase

144

10.2.1.2

Enzymes Responsible for Cellulose Degradation

145

10.2.1.3

Physical Pre-treatments to Break down Cellulose

145

10.2.2

Degradation of Hemicellulose

146

10.2.2.1

Enzymes Responsible for Degradation of Hemicellulose

146

10.2.2.2

Microbial Production of Hemicellulases

147

10.2.2.3

Physical Pre-treatments to Break down Hemicellulose

147

10.2.3

Degradation of Lignin

148

10.2.3.1

Microbial Production of Lignin Degrading Enzymes

148

10.2.3.2

Enzymes Responsible for the Degradation of Lignin

148

10.2.4

Degradation of Pectin

149

10.3

Utilizing Enzymes for the Degradation of Lignocellulosic Waste

150